About the Project
Our two aims in this project are
i) Purify ABCG2 from over-expressing cell lines to enable quantitative determination of transporter:substrate interaction using biophysical techniques
ii) Aim to identify the regions of the transporter responsible for drug binding by mutagenesis and functional activity
Through the project the student would receive training in a number of different techniques including molecular biology, cell culture, fluorescence-based assays, confocal microscopy, molecular modelling, membrane and protein biochemistry. The student would also receive training in data analysis, reading the scientific literature, and scientific writing and presentation. The student would contribute to a vibrant research group, and would make presentations to the group and the wider scientific community.
The University of Nottingham is one of the world’s most respected research-intensive universities, ranked 8th in the UK for research power (REF 2014). Students studying in the School of Life Sciences will have the opportunity to thrive in a vibrant, multidisciplinary environment, with expert supervision from leaders in their field, state-of-the-art facilities and strong links with industry. Students are closely monitored in terms of their personal and professional progression throughout their study period and are assigned academic mentors in addition to their supervisory team. The School provides structured training as a fundamental part of postgraduate personal development and our training programme enables students to develop skills across the four domains of the Vitae Researcher Development Framework (RDF). During their studies, students will also have the opportunity to attend and present at conferences around the world. The School puts strong emphasis on the promotion of postgraduate research with a 2-day annual PhD research symposium attended by all students, plus academic staff and invited speakers.
Wong, K., Briddon, S. J., Holliday, N. D., and Kerr, I. D. (2016) Plasma membrane dynamics and tetrameric organisation of ABCG2 transporters in mammalian cells revealed by single particle imaging techniques, Biochim Biophys Acta 1863, 19-29.
Horsey, A. J., Cox, M. H., Sarwat, S., and Kerr, I. D. (2016) The multidrug transporter ABCG2: still more questions than answers, Biochem Soc Trans 44, 824-830.
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