About the Project
Projects are available to further our understanding of how transcription factors, either individually or in combination, interact with the genome to initiate and maintain the activities of gene regulatory elements, in the context of both normal differentiation and also during cell fate changes in cancer. This may reveal new therapeutic opportunities.
Training/techniques to be provided:
Training will be provided in basic biochemical, molecular and cell biological approaches. This will involve cell culture and manipulation using CRISPR technologies, western blotting, recombinant protein production, nucleic acid isolation (eg RNA) and microscopy approaches to image dynamic regulatory events at the single cell level. However, it is important to take genome-wide views of the regulatory events, so bioinformatics training will be provided to analyse and integrate complex datasets. Publically available datasets will be used alongside those generated by the student from a range of possible approaches including RNA-seq, ChIP-seq, ATAC-seq, PRO-seq, Hi-seq, and Capture-C which capture a range of regulatory events ranging to 3D regulatory element rearrangement through to the production of the RNA transcripts.
Candidates are expected to hold (or be about to obtain) a minimum first class honours degree (or equivalent) in a related area / subject. Candidates with laboratory experience in molecular and cell biology techniques are encouraged to apply. Additional training in bioinformatics approaches is desirable. A keen interest in studying gene regulatory mechanisms is essential.
For international students we also offer a unique 4 year PhD programme that gives you the opportunity to undertake an accredited Teaching Certificate whilst carrying out an independent research project across a range of biological, medical and health sciences. For more information please visit http://www.internationalphd.manchester.ac.uk
As an equal opportunities institution we welcome applicants from all sections of the community regardless of gender, ethnicity, disability, sexual orientation and transgender status. All appointments are made on merit.
 Rogerson C., Britton, E., Withey, S., Hanley, N., Ang, Y. and Sharrocks, A.D. (2019) Identification of a primitive intestinal transcription factor network shared between oesophageal adenocarcinoma and its pre-cancerous precursor state. Genome Research. 29(5):723-736.
 Baker, S.M., Rogerson, C., Hayes, A., Sharrocks, A.D. and Rattray, M. (2019) Classifying cells with Scasat, a single-cell ATAC-seq analysis tool. Nucleic Acids Research. 47 (2), e10.
 Britton, E., Rogerson, C., Mehta, S., Li, Y., Fitzgerald, R., Ang, Y., and Sharrocks, A.D. (2017) Open chromatin profiling identifies AP1 as a transcriptional regulator in oesophageal adenocarcinoma. Plos Genetics, 13(8):e1006879.
 Yang, S-H., Kalkan, T., Morissroe, C., Marks, H., Stunnenberg, H., Smith, A., and Sharrocks, A.D. (2014) Otx2 and Oct4 drive early enhancer activation during ES cell transition from naïve pluripotency. Cell Reports. 7:1968-81
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