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Understanding the role of spliceosome gene mutations in disease


Project Description

The DNA of a cell is copied into a pre-messenger RNA (pre-mRNA) that the cell uses as a template for protein production. Some of the information contained in DNA is not required for making proteins, therefore, unwanted information must be removed before a protein is made. This unwanted information is removed, or spliced, from pre-mRNA by a process similar to the editing of unwanted frames from a film. This splicing of the pre-mRNA is very important because it must occur accurately in order for functional proteins to be produced. Splicing at the wrong position could have disastrous effects on the final protein produced. Mistakes in splicing could cause defects in the development of an organism or result in disease.

Pre-messenger RNA splicing is carried out by the spliceosome, a large RNA/protein complex composed of five small nuclear ribonucleoprotein particles (snRNPs). There are now ever increasing examples of mutations in snRNP proteins and other splicing factors being identified as the cause of diseases, including cancer. However, there is a lack of information on how these mutations influence the mechanisms of splicing and how this relates to the splicing events that are mis-regulated during the disease. We have identified mutations in a number of genes coding for snRNP proteins and splicing factors that cause specific diseases. However, it is unclear how these mutations affect splicing and cause the specific diseases. The project will use genetic, biochemical and molecular techniques to understanding the link between splicing factor gene mutation and splicing programmes that are disrupted to cause disease. This work will address a key fundamental question in bioscience for health which will have wide interest and impact.

Candidates are expected to hold (or be about to obtain) a minimum upper second class honours degree (or equivalent) in Genetics, biochemistry or Molecular Biology. Candidates with experience in Gene Expression analysis or with an interest in RNA processing are encouraged to apply.

Funding Notes

This project has a Band 2 fee. Details of our different fee bands can be found on our website (View Website). For information on how to apply for this project, please visit the Faculty of Biology, Medicine and Health Doctoral Academy website (View Website).

Informal enquiries may be made directly to the primary supervisor.

References

Jenkinson, EM, et. al., O’Keefe, R.T., Crow, YJ (2016) Mutations in SNORD118 cause the cerebral microangiopathy leukoencephalopathy with calcifications and cysts. Nature Genetics. 48(10):1185-1192

de Almeida RA, Fraczek MG, Parker S, Delneri D, O'Keefe RT. (2016) Non-coding RNAs and disease: classical ncRNAs make a comeback. Biochem Soc Trans. 44(4):1073-8

Wieczorek et al. (2014) Compound Heterozygosity of Low-Frequency Promoter Deletions and Rare Loss-of-Function Mutations in TXNL4A Causes Burn-McKeown Syndrome. American Journal of Human Genetics. 95(6):698-707

Hogg, R., de Almeida, RA., Ruckshanthi, J.P.D., and O’Keefe, R.T. (2014). Remodelling of U2-U6 snRNA helix I during pre-mRNA splicing by Prp16 and the nineteen complex protein Cwc2. Nucleic Acids Research. Nucleic Acids Research. 42:8008-23

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