Proteolysis is a tightly controlled regulatory step in many biological processes. Viral proteases are essential for the production of infectious viral progeny. Flaviviral proteases (like Zika and Dengue proteases) are good models for studies of regulations of proteolysis since the NS3 protease needs NS2B peptide for its folding and activity, NS3 is embedded in NS3 protein together with NTPase, RNA 5′ triphosphatase and helicase domains and has many protein-protein interaction partners.
Studies of viral proteases will be focused mainly on flaviviral proteases as model enzymes for enzyme activation, inhibition, folding, allosteric modulation, protein design, the interplay between different enzyme activities in multidomain proteins.
The work will include cloning, protein expressions, purifications, biophysical characterizations, and crystallizations, handling with small-compounds libraries and biological libraries, tissue culture work and analyses (microscopy, flow cytometry), development and adaptation of enzymological and phenotypic assays.
The outputs will contribute to the understanding of principles and consequences of down- and up-regulation of proteolysis.
How to apply
To apply for a PhD study at IOCB Prague, you must hold a Master’s degree (MSc) or the equivalent of the MSc in similar field (four or five year undergraduate degree). The application can be submitted before obtaining the Master’s degree, however, the applicant should obtain the degree within five months after the application deadline.