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Visualization with plasmonic nanoparticles using single molecule microscopy techniques


Department of Biophysical Chemistry

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Mrs Vladimíra Petráková No more applications being accepted Competition Funded PhD Project (Students Worldwide)
Prague Czech Republic Physical Chemistry Solid State Physics Synthetic Chemistry

About the Project

We offer fully funded PhD position at Heyrovsky Institute of Physical Chemistry, Prague, Czech Republic starting in September 2021. The position is part of a five-year project developing a new method for spatial manipulation of light at the nanoscale by assembly of plasmonic nanostructures. We aim to use this method to advance visualization of densely packed biomolecules and their dynamics.

The focus of the work will be on evaluating the magnitude of the fluorescence shifts and enhancement of plasmon-coupled fluorophores using single molecule localization microscopy and time-resolved single molecule spectroscopy. Single molecule localization microscopy overcomes the diffraction limit by calculating the center positions of a fluorescent spot based on the known point spread function of the optical microscope. While plasmonic coupling is known to strongly increase the number of emitted photons from a fluorophore, it also appears to affect the position of the emission. The work will consist of visualization of assembled fluorophores with and without plasmonic nanoparticles, data analysis and statistics. The candidate will choose one of the Ph.D. programs at University of Chemistry and Technology or at Czech Technical University in Prague, Czech Republic.

We expect the candidate will hold a Master degree (or equivalent) in life sciences, chemistry, physics, or engineering before assuming the position. An ideal candidate should be curious and enthusiastic to learn about superresolution microscopy. We value self-motivation and the ability to think independently. Good communication and time management skills are vital. Previous experience with nanoparticles, self-assembly, or microscopy techniques is an advantage.

If interested, please send your CV and motivation letter to [Email Address Removed] before March 15, 2021. For questions, please,contact Vladimíra Petráková [Email Address Removed].

Please, write in the subject line of your email SC2021_03.

 

Details of the PhD topic:

Visualization with plasmonic nanoparticles using single molecule microscopy techniques

The focus of the work will be on evaluating the magnitude of the fluorescence shifts and enhancement of plasmon-coupled fluorophores using single molecule localization microscopy and time-resolved single molecule spectroscopy. Single molecule localization microscopy overcomes the diffraction limit by calculating the center positions of a fluorescent spot based on the known point spread function of the optical microscope. While plasmonic coupling is known to strongly increase the number of emitted photons from a fluorophore, it also appears to affect the position of the emission. The work will consist of visualization of assembled fluorophores with and withoutz plasmonic nanoparticles, data analysis and statistics.

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