About the Project
Hypothesis and aims.
Here we hypothesise that cell-ECM interaction leads to the activation of pro-survival signalling pathways when the cells experience glucose deprivation. To investigate this we will:
1. Further characterise how cells respond to different glucose concentrations and what the ECM contribution is
2. As we already know that mTOR signalling is activated under glucose starvation in the presence of ECM, we will use this to identify the best time points to analyse changes in cell signalling
3. Use a phospho-proteomic approach coupled with mass spectrometry to identify the signalling pathways that are specifically up-regulated by the presence of ECM under glucose deprivation. The role of these will be further validated by analysing ECM-dependent cell growth when the identified pathways are inhibited
4. Use human breast cancer tissue sample to assess whether the identified pathways are up-regulated during breast cancer progression
Science Graduate School
As a PhD student in one of the science departments at the University of Sheffield, you’ll be part of the Science Graduate School. You’ll get access to training opportunities designed to support your career development by helping you gain professional skills that are essential in all areas of science. You’ll be able to learn how to recognise good research and research behaviour, improve your communication abilities and experience the breadth of technologies that are used in academia, industry and many related careers. Visit http://www.sheffield.ac.uk/sgs to learn more.
All applicants should ensure that both references are uploaded onto their application as a decision will be unable to be made without this information.
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