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Who are the important environmental producers and cyclers of DMSP? (TODDUBIO19ARIES)


Project Description

Introduction
Dimethylsulfoniopropionate (DMSP) is one of Earth’s most abundant organosulfur molecules. It is an anti-stress compound that is produced by marine phytoplankton and many marine bacteria. When released into the environment, DMSP has important roles in global nutrient and sulfur cycling, signaling pathways and in climate, since DMSP catabolism can generate the climateactive gas dimethylsulfide (DMS). There are few molecular-genetic studies into DMSP synthesis by any organism or environment. We recently identified the key genes involved in DMSP synthesis in bacteria and phytoplankton, thus are well placed to rectify this.

This project will:
- Explore the role of microbes in DMSP cycling at a world-renowned sampling station in the English Channel using cutting edge molecular techniques.

Methodology
You will use traditional oceanographic sampling combined with molecular ecology techniques, including gene-probing, metagenomics and metatranscriptomics, to study microbial populations that produce and catabolise DMSP in surface ocean samples from the English Channel (www.westernchannelobservatory.org.uk) over six-month study. The expression and abundance of the key genes will be assayed by PCR-based techniques. DMS and DMSP will be analysed by chromatography and mass spectroscopy-based methods to quantify rates of DMSP synthesis and catabolism. You will combine geochemical and molecular approaches to identify key microbes that produce and catabolise DMSP, identify the key genes and cognate pathways that contribute to the flux of these influential molecules. Culture-dependent studies on model microbes that make DMSP will enable the investigation of conditions that affect DMSP production. This project will provide essential data to enable scientists to better understand key biotransformations in the global sulfur cycle.

Training
You will receive exceptional training in molecular ecology and microbiology, bioinformatics, analytical chemistry, ecosystem modelling and in writing publications and will give presentations at international conferences. You will undertake research at UEA and Plymouth Marine Laboratory, gaining experience of working on-board a research ship during coastal marine fieldwork. You will learn good laboratory practice, quality assurance, and receive health and safety training.

Person specification
We require a committed student keen to master a wide range of techniques on this multidisciplinary project, who has experience in some of the key components.

Start Date: October 2019
Mode of Study: Full-time or Part-time
Studentship length: 3.5 years
Minimum entry requirement: UK 2:1

Funding Notes

This project has been shortlisted for funding by the ARIES NERC Doctoral Training Partnership. Undertaking a PhD with ARIES will involve attendance at training events.
ARIES is committed to equality & diversity, and inclusion of students of any and all backgrounds.
Applicants from quantitative disciplines with limited environmental science experience may be considered for an additional 3-month stipend to take appropriate advanced-level courses. Usually only UK and EU nationals who have been resident in the UK for 3 years are eligible for a stipend. Shortlisted applicants will be interviewed on 26th/27th February 2019.

Further information: View Website or contact us:

References

1. Curson ARJ, Todd, JD, Sullivan MJ and Johnston AWB (2011). Catabolism of dimethylsulfoniopropionate: microorganisms, enzymes and genes. Nature Reviews Microbiology 9: 849-859.
2. Curson ARJ, Liu J, Bermejo Martínez A, Green RT, Chan Y, Carrión O, Williams BT, Zhang SH, Yang GP, Page PCB, Zhang XH, Todd JD (2017). Dimethylsulphoniopropionate biosynthesis in marine bacteria and identification of the key gene in this process. Nature Microbiology. 2:17009.
3. Curson ARJ, Williams BT, Pinchbeck BJ, Sims LP, Martínez AB, Rivera PPL, Kumaresan D, Mercadé E, Spurgin LG, Carrión O, Moxon S, Cattolico RA, Kuzhiumparambil U, Guagliardo P, Clode PL, Raina JB, Todd JD. (2018). DSYB catalyses the key step of dimethylsulfoniopropionate biosynthesis in many phytoplankton. Nature Microbiology. 4: 430-439.
4. Archer, S.D., Kimmance, S.A., Stephens, J.A., Hopkins, F.E., et al., 2013. Contrasting responses of DMS and DMSP to ocean acidification in Arctic waters. Biogeosciences 10, 1893-1908.
5. Todd JD, Rogers R, Li YG, Wexler M, Bond PL, Sun L, Curson AR, Malin G, Steinke M, Johnston AWB. (2007). Structural and regulatory genes required to make the gas dimethyl sulfide in bacteria. Science. 315: 666-9.

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