Dynamic localisation of the bacterial Sec machinery

The Sec machinery is responsible for the transport of proteins across the cytoplasmic membrane in most organisms. In bacteria, the core Sec machinery is evolutionarily conserved and consists of the proteins SecA, SecY, SecE, SecG, SecD, SecF, YidC and YajC. Despite its important role in localising other proteins to the correct compartment, very little is known about the localisation of the Sec machinery itself. Depending on the Sec component and the species, Sec proteins are distributed in the cytoplasmic membrane, localised to specific patches or arranged into structures such as helices. Very little work has investigated the dynamics of these structures and how changes to the environment affect these dynamics or the structures themselves. This research will investigate the localisation of different components of the Sec machinery in the model Gram-negative bacterium Escherichia coli using a range of microscopic methods, including cutting-edge ultra-high-resolution microscopy approaches (di Maio). The student will also use a range of modern methods in molecular genetics and protein biochemistry methods (Huber).

At the University of Birmingham, Institute of Microbiology and Infection, you will be part of a vibrant and lively postgraduate community and one of the world-leading institutes for microbiological and infectious disease research.

Person Specification
If you are an enthusiastic scientist with an interest in the molecular biology of fundamental biological processes and are looking for a PhD position, we would be happy to hear from you. A background in immunology, microbiology or molecular genetics would be helpful. An enthusiastic approach to science, together with the ability to take ownership of your own project within a young, dynamic research team, is essential. You should have a commitment to academic research and hold or realistically expect to obtain at least an Upper Second Class Honours Degree in a relevant subject.