Ferritins as nano-cage bioreactors for synthetic biology applications (LEBRUNU19DTP2)

The cellular iron storage protein ferritin is composed of 24 subunits, arranged in a highly symmetric fashion to form an approximately spherical protein shell surrounding a hollow centre in which large amounts of iron can be stored as a ferric-oxy-hydroxide mineral. The ferritin protein shell can potentially accommodate a range of other molecules, and the aim of this project is to use synthetic biology approaches to develop bacterial and plant ferritins as microaerobic nano-cage bioreactors, through the encapsulation of proteins and peptides for biotechnological applications. Methodologies for peptide and protein encapsulation will be developed, with eventual targets including technologically relevant enzymes such as FeFe hydrogenase, which catalyses the reduction of protons to hydrogen gas. This reaction/enzyme is of great current interest because of its potential for large scale generation of clean energy. This multi-disciplinary project will be based in the Le Brun lab at UEA and the Balk lab at John Innes Centre and will involve protein purification, biochemistry, spectroscopy and kinetics, offering excellent training potential for the appointed student within a supportive and stimulating environment.

This project has been shortlisted for funding by the Norwich Biosciences Doctoral Training Partnership (NRPDTP). Shortlisted applicants will be interviewed as part of the studentship competition. Candidates will be interviewed on either the 8th, 9th or 10th January 2019.
The NRP DTP offers postgraduates the opportunity to undertake a 4-year research project whilst enhancing professional development and research skills through a comprehensive training programme. You will join a vibrant community of world-leading researchers. All NRPDTP students undertake a three-month professional internship (PIPS) during their study. The internship offers exciting and invaluable work experience designed to enhance professional development. Full support and advice will be provided by our Professional Internship team. Students with, or expecting to attain, at least an upper second-class honours degree, or equivalent, are invited to apply.

For further information and to apply, please visit our website: www.biodtp.norwichresearchpark.ac.uk

Project Start Date: 01/10/2019
Length of Studentship: 4 years
Mode of Study: Full-Time
Minimum Entry Requirements: UK 2:1 & Eng Lang (6.5 overall, 6 in each section)