NADPH oxidase-dependent regulation of platelets and its relevance in haemostasis and thrombosis

NADPH oxidases are the most important enzymes in the redox balance of platelets. The generation of superoxide anion by NADPH oxidase is involved in the normal functioning of platelets and in their hyperactivity in cardiovascular pathologies. In this studentship, we will investigate the molecular mechanisms linking NADPH oxidase and platelet activation. We will utilise a variety of functional techniques (including aggregation, flow cytometry, whole blood flow assays, etc.) and biochemical approaches (including intracellular calcium analysis, reactive oxygen species detection, molecular signalling, etc.). In addition to working with human blood, the student will have access to animal models for the study of thrombotic responses in genetically modified mice (NOX1-/- and NOX2-/-).

The ideal candidate will have previous experience with biochemical techniques (such as immunoblotting and flow cytometry) and blood cell isolation. Previous experience in the haemostasis and thrombosis filed and a background in animal handling will be desirable but not essential.