Hypoxia-dependent angiogenesis: the role of NADPH oxidases.

Angiogenesis (i.e. generation of new blood vessels in living tissues) is a highly dynamic process that regulates vascular homeostasis and is essential for tissue repair. Although hypoxia (i.e. reduced oxygen levels) is a strong pro-angiogenic stimulus in healthy tissues, in ischemic diseases such as myocardial infarction and peripheral artery disease, hypoxia is not sufficient to stimulate angiogenesis and maintain circulatory homeostasis. To understand and treat ischemic diseases, it is paramount to understand how hypoxia regulates angiogenesis. Reactive oxygen species (ROS) have been shown to play a critical role in the angiogenic responses stimulated by hypoxia. NADPH oxidases (NOXs) play a key role in the generation of ROS in hypoxic tissues and are significantly increased in vascular endothelial cells by hypoxia. In this study we will investigate the mechanisms regulating NOX expression in endothelial cells and their role in hypoxia-induced angiogenesis.

The ideal candidate will have previous experience with biochemical techniques (such as immunoblotting and flow cytometry) and cell culture. Previous experience in molecular signalling research and a background in animal handling will be desirable but not essential. This project will take place within the Advanced Hypoxic Imaging Facility of the University of Bath.