Fundamental insight and biotechnological optimization to enable commercial production of renewable propane

BBSRC-funded iCASE PhD-studentship on “Fundamental insight and biotechnological optimization to enable commercial production of renewable propane”

Very recently, we have on our own and together with Prof. Nigel Scrutton at Univ. Manchester described 5 novel synthetic pathways1, 2 that enable renewable biosynthesis of propane for the first time, a highly attractive fuel product with an existing global market. In order to proceed towards application, however, we first need to improve the biocatalytic process. The initial emphasis in this PhD-project is placed on fundamental understanding, later shifting to practical implementation and scale-up. The primary metabolic engineering research targets include enhancing the (i) supply of metabolic precursor and (ii) alkane synthesis reaction. The work is carried out as an integrated collaborative project involving BDS Fuels, C3 Biotechnologies, as well as Univ. Manchester. In London, we will study native alkane synthesis systems by reconstitution and biochemical analysis. Precursor flux in an E. coli model system will be investigated by a combination of native and synthetic pathway engineering. These efforts will be combined with complementary studies at Univ. Manchester. To enhance the overall system we will also develop and carry out computational pathway balance analysis. Final laboratory production strains will be evaluated together with BDS Fuels.

A broad set of methodologies will be learned and utilised in the project including computational modelling, recombinant protein synthesis and purification, synthetic operon and pathway construction, metabolite pool and metabolic flux quantification, targeted SRM protein quantification. We will also support and encourage the student to participate in local and national events that explore new start-up ventures. The student will also have an opportunity to participate in Horizon 2020 consortium meetings (www.photofuel.eu).

Requirements and eligibility:
We seek a highly motivated candidate with a drive to make a difference. Knowledge of and experience with microbiology, molecular biology and biochemistry are essential. The position is well-suited for students with an interest to pursue a career in industrial biotechnology. Students are expected to hold a BSc degree in Biochemistry/Biology or similar, at 2:1 level or better, and preferably should also hold or expect to obtain by October 2016, a Masters degree in Biotechnology or Synthetic Biology (at Merit level at least). Experience or studies in business, experience with synthetic biology (i.e. iGEM) and/or internships at biotechnology companies are highly valued.

Deadline for applications is 30th June 2016 but applications will be considered as they are received so early applications are encouraged. A full CV, a personal statement and full contact details of 2 academic referees should be submitted to Dr Patrik Jones ([Email Address Removed])