Characterising the Microbe-Mineral Interface of the Biomining Bacteria Acidithiobacillus (CLARKEU17DTP)

The microorganism Acidithiobacillus ferrooxidans is an acid-loving, iron oxidising bacteria that can grow on mineral ores in the presence of oxygen. These bacteria break down the mineral pyrite, causing the release of soluble metal ions and sulfates from the ore, causing the production of acidic iron containing water that can lead to serious river and lake pollution (acid mine drainage). Conversely, these bacteria can also be used to extract copper from low grade copper ores in a process known as bioleaching or biomining. Currently 20 % of the worlds copper production comes from this bioleaching, an energy efficient process that avoids the use of toxic chemicals.

Iron oxidation by A. ferrooxidans occurs at the surface of the cell, but the protein responsible appears too small to form an effective electron channel through the outer cell membrane. This project aims to investigate how this outer membrane protein can oxidise pyrite and pass the electrons through the outer membrane and into the cell. The student will use a range of structural and kinetic methods, including small angle scattering and proteoliposome systems, to properly characterize a recombinant form of the cytochrome, and use these results to better understand how it functions to oxidise iron at the surface of the bacterium.

This project has been shortlisted for funding by the Norwich Biosciences Doctoral Training Partnership (NRPDTP). Shortlisted applicants will be interviewed as part of the studentship competition. Candidates will be interviewed on either the 10th, 11th or 12th January 2017.

The Norwich Biosciences Doctoral Training Partnership (NRPDTP) offers postgraduates the opportunity to undertake a 4 year research project whilst enhancing professional development and research skills through a comprehensive training programme. You will join a vibrant community of world-leading researchers. All NRPDTP students undertake a three month professional internship (PIPS) during their study. The internship offers exciting and invaluable work experience designed to enhance professional development. Full support and advice will be provided by our Professional Internship team. Students with, or expecting to attain, at least an upper second class honours degree, or equivalent, are invited to apply.

For further information and to apply, please visit our website: www.biodtp.norwichresearchpark.ac.uk