A dynamic role for the sodium pump in amyotrophic lateral sclerosis

Na/K exchange pumps are ubiquitously expressed proteins that have been associated with diseases of the motor system including PD and ALS. However, the link between sodium pump dysfunction and disease progression is unknown. Sodium pumps play dynamic roles in regulating spinal motoneuron output due to α3 subunit containing pumps. These have a low affinity for intracellular Na+ and hence are only recruited during intense neural activity to homeostatically hyperpolarize the membrane potential. The underlying mechanism is highly conserved having been described in Xenopus tadpoles and in neonatal mouse. This project will use genetic zebrafish models of ALS to unravel the contribution of α3 subunits (and α2 expressed in neurons and glia) and to track ALS disease progression at the motoneuron and premotor circuit levels. The project, which will involve collaboration with the Becker group at the University of Edinburgh, will provide a broad training in neuroscience techniques ranging from patch clamp electrophysiology of spinal neurons, to molecular genetic manipulations and the developmental expression of specific pump protein subunits.

Prerequisites
Ideally a suitable student will have an honours degree or equivalent in Neuroscience or a related discipline and an understanding of neuronal physiology and molecular genetic approaches. Essential skills would include proficiency in laboratory techniques and analytical methods that can be applied to these areas and the enthusiasm and ability to learn quickly across these areas. Although not essential a Masters in the same area would be an advantage.
Please contact your intended supervisor to discuss the project and your suitability for it before submitting your application.

The project is a part of SPRINT-MND/MS, a new Scotland-wide PhD scheme for research into motor neurone disease and multiple sclerosis. Projects, encompassing a wide range of topics including laboratory, clinical, and social sciences, are available at Aberdeen, Dundee, Edinburgh, Glasgow and St Andrews Universities. This exciting initiative provides a great opportunity for budding researchers in any field related to MND or MS to join Scotland’s network of world-leading scientists and health professionals. Find more information here: http://www.edneurophd.ed.ac.uk/sprint-mndms-phd-programme