Investigating Cell and Extracellular Matrix Interactions within the Bone Marrow Stem Cell Niche

Medical research strategies such as tissue engineering and regenerative medicine aim to generate functional tissue analogues for therapeutic use. In order to fully reproduce the complexity of biological systems such implants require the reconstruction of biological complexity at high and low length-scales. The majority of such strategies focus on bulk macroscopic structure but can be limited in their ability to effectively reproduce cellular microenvironments. We have developed a novel technique which utilises laser capture technology to pattern individual cells and structural elements in 3D enabling the study of biology at a length scale that has previously been unachievable (Kirkham GR et al “Precision Assembly of Complex Cellular Microenvironments using Holographic Optical Tweezers”. Scientific Reports, 5 (2015)).

This project will use this system to study the interactions between cells of the bone marrow stem cell niche and their physical environment. This will involve an analysis of the interactions between these cells and components of the extracellular matrix using the 3D micromodel system as well as bespoke 2D models. This multidisciplinary work will be developed in collaboration with the Universities of Nottingham and Glasgow and this project will be conducted both at NTU and with Dr Lee Buttery at the University of Nottingham.

Specific qualifications/subject areas required of the applicants for this project
(e.g. 2:1 BSc Hons. Chemistry):
UK First-class or 2:1 degree (or UK equivalent according to NARIC) in Biological/Biomedical Sciences, Biochemistry, Cell Biology, Biotechnology or a closely related discipline and/or a Masters degree with a minimum of a merit/commendation (or UK equivalent according to NARIC) in a relevant subject.