Novel transfection agents as tools for biology

Genetically engineered cells are valuable tools in cell biology and biotechnology. The ability to express foreign genes (such as GFP) within cells is critical to modern biological research. However, current methods to engineer cell lines have several disadvantages, including the cost associated or the stress induced in the cell. As such, new materials and technologies are required that can efficiently transport genetic information across cellular barriers without damaging these cell lines.

We have recently developed an innovative screening technology, that allow us to identify transfection agents in a timely and efficient way. This methodology allow us to screen for transfection efficiency and toxicity without having to purify any of the candidate transfection agents, simplifying this way the discovery process. In this multidisciplinary project, we will identify novel transfection agents capable of delivering plasmid DNA to cells that are classically thought of as difficult or impossible to transfect (such as macrophages or neuronal cells).

This project is a collaboration between Dr Francisco Fernandez-Trillo, Prof. Robin May and Dr Sara Jabbari, an it will combine in polymer synthesis and characterisation, cell biology and computational sciences.

For further details about the project, please contact Dr Fernandez-Trillo ([Email Address Removed]).