Is mutational meltdown a threat to the mega diverse genus Begonia?

Interested candidates should contact Dr Kidner direct ([Email Address Removed])

Worldwide herbaria contain millions of specimens collected over the past 400 years. Accessing the genetic information in these specimens has been problematic due to the poor quality of DNA in dried and aged plant material. Recently we have shown that targeted capture provides a reliable means to recover hundreds of kilobases of sequence from specimens with even very low amounts of degraded DNA. This sequence can be used for identification, phylogenetics, population genetics and for functional studies. This project will develop this technique to provide information on the genetic health of populations. Begonia is one of the five largest angiosperms genera. It is found throughout the tropics in low land montane forests. It is also the basis of an important horticultural industry. One of the explanations for the specious nature of Begonia is the typically small, endemic populations. These are particularly vulnerable to extinction through loss of habitat but also through mutational meltdown. We have developed a set of hybrid baits for Begonia. This project will use these baits to capture sequence from herbarium specimens, including type specimens of widespread, local and very local (single site) species. We will confirm the identification of the specimens and determine the level of heterozygosity in each specimen as a measure of genetic health. Specimens collected over long time periods (initial nineteenth century collections and recent re-collections) will be compared to assess how the health of the populations has changed over time. The objective of the project will be to determine the utility of herbarium specimens for assessing the genetic health of species