Prof Graham Pavitt, Dr D Talavera, Prof Raymond O'Keefe
No more applications being accepted
Competition Funded PhD Project (European/UK Students Only)
About the Project
Eukaryotic genomes are extensively transcribed generating many different RNAs with no known function. Gene expression is regulated at multiple levels in response to environmental stresses, including at the level of protein synthesis (reviewed in ref 1). One under-researched area is how non-protein coding RNAs (ncRNAs) contribute to translational controls in the model eukaryote yeast Saccharomyces cerevisiae. The project will address this question. It will involve the student undertaking a mix of computational and lab experiments including generating and analysing high-throughput RNA sequencing data sets to examine the association of ncRNAs with actively translating ribosomes and then studying experimentally their impact on translational controls in response to a range of environmental stresses. The project will build on existing strengths of the supervisory team and make use of various laboratory complementary expertise and resources including a recently generated series of 1502 molecular barcoded ncRNA gene deletion strains encompassing a wide range of ncRNAs for high-throughput functional analyses (ref 2).
This project represents an ideal opportunity for an individual interested in understanding RNA-mediated mechanisms of gene expression control to gain a wide range of experimental and analytical skills and contribute to our understanding of this emerging area of biological research.
https://www.research.manchester.ac.uk/portal/graham.pavitt.html
https://www.research.manchester.ac.uk/portal/David.Talavera.html
https://www.research.manchester.ac.uk/portal/rokeefe.html
Funding Notes
This project is to be funded under the BBSRC Doctoral Training Programme. If you are interested in this project, please make direct contact with the Principal Supervisor to arrange to discuss the project further as soon as possible. You MUST also submit an online application form - full details on how to apply can be found on the BBSRC DTP website www.manchester.ac.uk/bbsrcdtpstudentships
Applications are invited from UK/EU nationals only. Applicants must have obtained, or be about to obtain, at least an upper second class honours degree (or equivalent) in a relevant subject.
References
1. Dever TE, Kinzy TG, Pavitt GD. (2016) Mechanism and Regulation of Protein Synthesis in Saccharomyces cerevisiae. Genetics. 203:65-107. doi: 10.1534/genetics.115.186221.
2. Parker S, Fraczek MG, Wu J, Shamsah S, Manousaki A, Dungrattanalert K, de Almeida RA, Estrada-Rivadeneyra D, Omara W, Delneri D, O'Keefe RT. (2017) A resource for functional profiling of noncoding RNA in the yeast Saccharomyces cerevisiae. RNA 23(8):1166-1171. doi: 10.1261/rna.061564.117.
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