Lab on a chip for pathogen analysis (University of Hull) - (ViBrANT ITN)

Available at Faculty of Science and Engineering, University of Hull (UK)
Supervisor: Prof. Nicole Pamme
Location: School of Mathematics and Physical Sciences (Chemistry), University of Hull (UK)

Background: Pathogen infection is a big burden in the developed as well as developing world affecting millions each year with symptoms ranging from mild discomfort to a threat to life. A large challenge in managing pathogen treatment is that fact that pathogen analysis is hampered by lengthy procedures, often involving selective culturing of samples that may take several days. One promising technique for faster pathogen analysis is fluorescence in-situ hybridization (FISH), which allows the identification of microbial cells by epifluorescence microscopy directly in a sample of interest in less than 3 h. However, due to low numbers of cells present in the clinical samples, pre-enrichment steps are required before the FISH procedure can be carried out, which results in full analysis taking one day.

Aim of project: In this project, we will investigate microfluidic-based methods for fast pathogen cell pre-enrichment in blood samples and subsequent trapping in defined locations for FISH analysis. Pathogen cell enrichment procedures will include inertial microfluidics, as well as magnetic or acoustic trapping forces. For analysis, we will employ biochemistry developed by one of the ITN partners (Biomode), based on peptide nucleic acid (PNA) or locked nucleic acid (LNA) which are more robust and specific compared to conventional DNA probes. Furthermore, in collaboration with our partners in Frankfurt, we will develop miniaturised platforms with immobilised adhesins to study binding and interactions to host-receptors. The post holder will be trained in a highly interdisciplinary manner, gaining experience of microfluidic device design and fabrication, physical principles of cell separation and bioanalysis of pathogens.

Requirements: The successful candidate must hold a BSc in Biochemistry/Chemistry or a related discipline and ideally an associated Masters or an equivalent level of professional qualifications or experience. Candidates must comply with both EU and the Hull University eligibility criteria.

Further details on this project are available from Professor Nicole Pamme ([Email Address Removed]). Further details about the Hull University PhD programme see (http://www.hull.ac.uk/Choose-Hull/Study-at-Hull/Admissions/Postgraduate/Entry-requirements.aspx) and the research group of Prof. Pamme are available at www.hull.ac.uk/chemistry/pamme and www.pammegroup.org.

We will contact you by 28.4. to tell you if you will be asked for an interview.
The interviews will take place on 1.5. via Skype at 9am - 5pm UK time.
The time will depend on your particular time zone.