Spatial control of autophagosome biogenesis
Autophagy is an essential catabolic process that promotes cell survival during episodes of nutrient/growth factor stress. Autophagy is involved in many human diseases, so complete understanding of this process is needed. During autophagy cytoplasm is sequestered into double membrane autophagosomes that traffic along microtubules to fuse with lysosomes. Our hypothesis is that autophagosome motility and positioning are crucial for the regulation of autophagosome biogenesis and maturation. This project will use live-cell imaging and in vitro motility assays to monitor and characterise microtubule-based autophagosome trafficking.
Betin, V.M.S. & Lane, J.D. (2009) Caspase cleavage of Atg4D stimulates GABARAP-L1 processing and triggers mitochondrial targeting and apoptosis. Journal of Cell Science 122: 2554-2566