Effects of transcription on Genome stability
Transcription-coupled DNA repair (TCR) pathways prioritise the repair of certain lesions in "active" genes. These pathways help maintain genome integrity throughout the lifetime of multi-cellular organisms, and thus help prevent the occurrence of mutation that might cause cancer or other disorders. In bacteria, TCR also helps prevent mutagenesis, but in some circumstances components of the TCR pathway promote the generation of antibiotic-resistant mutants.
Research in our lab is focused on understanding the effects of DNA damage on transcription, and of the molecular mechanisms of TCR. We work primarily with a bacterial model system, and use a combination of in vivo and in vitro assays to investigate the mechanisms by which RNA polymerases are removed from the site of damage, and the DNA repair process is accelerated. We also have interests in various other aspects of transcriptional control, DNA repair and synthetic biology, and prospective students should contact us for further details of possible graduate student projects to be offered in the forthcoming year.
Fan, J., Leroux-Coyau, M., Savery, N.J. and Strick, T.R. (2016) Reconstruction of bacterial transcription-coupled repair at single-molecule resolution. Nature, 536, pp. 234-237.
Haines, NM, Kim, Y-I, Smith, AJ & Savery, NJ 2014, ‘Stalled transcription complexes promote DNA repair at a distance’. Proceedings of the National Academy of Sciences of the United States of America, vol 111., pp. 4037-42