• University of Leeds Featured PhD Programmes
  • University of Glasgow Featured PhD Programmes
  • Carlos III Health Institute Featured PhD Programmes
  • University of Bristol Featured PhD Programmes
  • University of Mannheim Featured PhD Programmes
  • University of Leeds Featured PhD Programmes
  • University of Cambridge Featured PhD Programmes
  • London School of Economics and Political Science Featured PhD Programmes
Ludwig-Maximilians-Universität Munich Featured PhD Programmes
Imperial College London Featured PhD Programmes
University of Southampton Featured PhD Programmes
University of Oxford Featured PhD Programmes
National University of Singapore Featured PhD Programmes

Structure and function studies of isolated Z-discs from muscle

This project is no longer listed in the FindAPhD
database and may not be available.

Click here to search the FindAPhD database
for PhD studentship opportunities
  • Full or part time
    Prof Trinick
  • Application Deadline
    Applications accepted all year round
  • Competition Funded PhD Project (European/UK Students Only)
    Competition Funded PhD Project (European/UK Students Only)

Project Description

The Z-disc is the anchoring structure for the ends of thin (actin) filaments and transmits contractile force between adjacent muscle sarcomeres. The primary linkages between thin filament ends of opposite polarity here are Z-bridges composed of-actinin. In addition to have this purely mechanical function, the Z-disc is now known to contain ~40 different proteins and to have several other functions, particularly stress sensing into nuclear and proteolytic signalling pathways involving muscle growth and degradation. Because it has so far only been studied in the electron microscope in thin sections, the structure of the Z-disc is known only in outline, to a resolution of 4-5 nm, whereas ~2 nm resolution is required to recognise protein shapes and accurately dock crystal structures. There are reports of the preparation of isolated Z-discs from both invertebrate and vertebrate muscle 50 years ago, but such preparations have not been subjected to modern electron microscopy methods, such as cryo-EM or tomography. Isolated Z-discs are a valuable specimen for EM studies because they are a naturally thin and do not have to be cut into thin sections, which causes damage and loss of resolution. To obtain 3D models with improved resolution, this project will study isolated Z-discs from both invertebrate and vertebrate muscle, including cardiac. Isolated Z-discs are also valuable for compositional studies, such as monitoring gain and loss of components that are transient in vivo. In addition to its fundamental importance in biology, this project will provide an excellent training in modern electron microscopy (cryo-tomography, image processing) as well as a wide variety of biochemical methods.

Further details from Prof John Trinick ([email protected])
See also http://www.fbs.leeds.ac.uk/staff/profile.php?un=chbjat

Funding Notes

Competitive schemes are open in the Faculty of Biological Sciences at Leeds University, including BBSRC DTC and Wellcome Trust programmes.

How good is research at University of Leeds in Biological Sciences?

FTE Category A staff submitted: 60.90

Research output data provided by the Research Excellence Framework (REF)

Click here to see the results for all UK universities
Share this page:

Cookie Policy    X