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Analysis of USP9X in low grade ovarian serous carcinoma

Project Description

The Gorringe lab focuses on cancer precision medicine: applying genomics to clinical questions in breast and ovarian cancers.

The focus in this project is on ovarian cancer patients with low-grade serous carcinoma (LGSC), a subtype that tends to occur in younger women and does not respond to current standard chemotherapy. Our exome sequencing identified recurrent mutations in KRAS (18, 30%), BRAF (8, 13%) and NRAS (6, 10%), as expected. Additionally, 4 tumours (13%) had inactivating alterations in USP9X, a novel candidate tumour suppressor gene.

We will test the transformation potential of USP9X by gene knockdown in cell lines using lentiviral vectors. We will assay knock-down efficiency using quantitative RT-PCR and Western blot, and assess proliferation/apoptosis using methods developed by the Victorian Centre for Functional Genomics including high-content cellular imaging. We will measure clonogenic survival and anchorage independent growth (soft agar cloning).

Depending on the length of the degree course project, the project may also entail DNA sequencing of additional candidate genes identified by exome and whole genome sequencing, as well as tissue microdissection and DNA/RNA extraction.

Funding Notes

All PhD students at Peter Mac must have a scholarship from The University of Melbourne or through another government, trust or philanthropic organisation. Before applying for a scholarship, you must have agreed on a project with an institute supervisor.

For further information about the university application process, see:
View Website

For further information regarding scholarships (both local and international), see:
View Website
Closing dates for applications for scholarships to commence in 2017: Round 1 -31 October 2016; Round 2 - 18 Dec 2016.

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