Deciphering inhibitory activities towards deposition of epigenetic marks controlling ageing rate
Over two decades of intense research in the field of epigenetics have produced compelling evidence that chromatin modifications are crucial for processes such as DNA repair, transcription, splicing, mitosis, meiosis, and in the preservation of the epigenetic memory of cells. One of the best examples of a chromatin modification implicated in that sort of repertoire of functions is methylation at histone 3 on lysine 4 (H3K4).
This abundant and conserved modification is deposited through a complex called the MLL/SET/COMPASS complex comprising a core complex and Histone MethylTransferase (HMTs) enzymes. Interestingly, lowering the levels of methyl marks at H3K4 using RNAi directed at the core complex increases C. elegans lifespan.
We have recently investigated how the core complex contributes towards the deposition of methyl marks at H3K4 and unexpectedly revealed an inhibitory activity towards deposition. The core complex component responsible for this inhibitory activity is RBBP-5.
Your project will aim at characterising this novel activity using RNAi, genetics, and next generation sequencing technologies such as RNA-seq, ChIP-seq, and GRO-seq. At the end of your PhD, you will have developed an expertise of both wet lab and bio-informatics, which are used across disciplines and are in demand.
This project has a Band 3 fee. Details of our different fee bands can be found on our website. For information on how to apply for this project, please visit the Faculty of Biology, Medicine and Health Doctoral Academy website. Informal enquiries may be made directly to the primary supervisor.
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