• University of Pennsylvania Featured PhD Programmes
  • Aberdeen University Featured PhD Programmes
  • University of Cambridge Featured PhD Programmes
  • University of Tasmania Featured PhD Programmes
  • Staffordshire University Featured PhD Programmes
University of York Featured PhD Programmes
EPSRC Featured PhD Programmes
University of Leeds Featured PhD Programmes
University of Bristol Featured PhD Programmes
University of Reading Featured PhD Programmes

The role of Topoisomerase II beta Neuronal DNA repair in aging (HLS/SE/DRFAPP7P/63601)

This project is no longer listed in the FindAPhD
database and may not be available.

Click here to search the FindAPhD database
for PhD studentship opportunities
  • Full or part time
    Dr Padget
  • Application Deadline
    Applications accepted all year round

Project Description

Neurons show high levels of metabolic active which results in the production of oxidative stress. However this is counterbalanced by a robust repair system in these cells. The enzyme DNA topoisomerase II beta (Top2B) is required for double stand break repair in neurons and also influences repair of DNA damage by oxidative stress, thus it is a key player in maintaining genomic integrity of these cells. This process is important as many neurodegenerative diseases such as Alzheimer’s disease cause the accumulation of oxidative stress in their early stages, which if go unrepaired may lead to further aberrant neuronal cell function and death. However, several mammalian studies suggest that Top2B is down-regulated in aged neurons. So understanding the impact of this upon DNA repair is an important step in elucidating the effect of aging upon levels of DNA damage in these cells. Thus the focus of the project is to investigate whether down-regulation of Top2B in aged neurones is correlated with decreased levels of DNA repair and decreased transcription of key genes in these processes which may be influenced by the action of Top2B. In addition, cell lines models will be used to test whether epigenetic up regulation of Top2B levels can modulate repair mechanisms and transcription in neurons to determine whether this would be a useful potential strategy for cell therapy.

Informal Enquiries
Enquiries regarding this studentship should be made to: Dr Kay Padget [email protected]
For further details of how to apply, entry requirements and the application form, see
https://www.northumbria.ac.uk/research/postgraduate-research-degrees/how-to-apply/
Please ensure you quote the advert reference above on your application form.

Eligibility
For further details of how to apply, entry requirements and the application form, see
https://www.northumbria.ac.uk/research/postgraduate-research-degrees/how-to-apply/
Please ensure you quote the advert reference above on your application form.

How to Apply
For further details of how to apply, entry requirements and the application form, see
https://www.northumbria.ac.uk/research/postgraduate-research-degrees/how-to-apply/
Please ensure you quote the advert reference above on your application form.


Funding Notes

This studentship is only open to self-funding candidates. Self-funding candidates are expected to pay University fees and to provide their own living costs. In addition, a ‘bench fee’ will have to be paid to cover project running costs (at a level that will be determined specifically for each project).

References

MRE11 facilitates the removal of human topoisomerase II complexes from genomic DNA. Lee KC, Padget K, Curtis H, Cowell IG, Moiani D, Sondka Z, Morris NJ, Jackson GH, Cockell SJ, Tainer JA, Austin CA. (2012) Biol Open. Sep 15;1 (9):863-73.
Histone deacetylase inhibition redistributes topoisomerase IIβ from heterochromatin to euchromatin. Cowell IG, Papageorgiou N, Padget K, Watters GP, Austin CA. (2011) Nucleus. Jan-Feb;2(1):61-71.

Share this page:

Cookie Policy    X