• Aberdeen University Featured PhD Programmes
  • Staffordshire University Featured PhD Programmes
  • University of Tasmania Featured PhD Programmes
  • University of Pennsylvania Featured PhD Programmes
  • FindA University Ltd Featured PhD Programmes
  • University of Cambridge Featured PhD Programmes
University of York Featured PhD Programmes
University of Bristol Featured PhD Programmes
Coventry University Featured PhD Programmes
University of Leeds Featured PhD Programmes
University of Reading Featured PhD Programmes

Expansion of salivary gland stem cells for autologous stem cell transplantation

Project Description

Oral dryness is a common side effect from irradiation of head and neck cancers. Regeneration of salivary glands (SGs) is the most likely remedy since artificial salivas and optimising irradiation protocols only have limited benefits. In vitro expansion of SG stem cells is key for autologous stem cell transplants since only small amounts of SG tissue can be obtained through biopsies to isolate and grow stem cells. Thus far, enrichment in stem cells has been obtained through in vitro culture of SG tissue in primary spheres known as salispheres. However, salispheres rapidly differentiate in vitro, restricting the potential to expand SG stem cell populations. The aim of this project is to improve the in vitro culture of mouse adult SG stem cells to obtain higher yields required to improve the SG function after radiotherapy.

The project will use a range of transgenic mice using the inducible Cre-LoxP technology to monitor activation of signalling pathways, trace and isolate specific cell lineages, and modulate signalling pathways both in vivo and in salispheres in vitro. The techniques used in this project include state-of-the-art mouse genetics, sphere culture of SG cells, cell sorting by flow cytometry, confocal microscopy and immunostaining. In the first year of the PhD, the student will attend taught courses including Experimental Approaches, Project Design and Stem Cell Biology.

The student will join two research groups with complementary expertises in mouse genetics (Miletich) and in vitro culture of SG stem cells (Carpenter).

How good is research at King’s College London in Allied Health Professions, Dentistry, Nursing and Pharmacy?

FTE Category A staff submitted: 52.95

Research output data provided by the Research Excellence Framework (REF)

Click here to see the results for all UK universities

Email Now

Insert previous message below for editing? 
You haven’t included a message. Providing a specific message means universities will take your enquiry more seriously and helps them provide the information you need.
Why not add a message here
* required field
Send a copy to me for my own records.
Email Sent

Share this page:

Cookie Policy    X