Investigation into the potential of using different delivery systems of cultured oral keratinocytes as a possible treatment for mucosal defects

The overall aim of the project is to investigate the possibility of being able to remove a small biopsy of a patients oral mucosa from a suitable site, expand the numbers of keratinocytes in culture, before seeding these cells onto a suitable delivery system and reimplanting into the patient over their mucosal defect.

The project will involve establishing cultures of human oral keratinocytes from different sites within the mouth, growing these cells on feeder layers of fibroblasts before digestion of the cells and reseeding onto different delivery substrates. To determine the feasibility of such an approach, keratinocyte expression of cytokeratins will be assessed both before and after seeding onto the delivery substrates using immunohistochemistry and RT-PCR, to examine whether the cells’ phenotype has been maintained from the particular isolation site. These delivery substrates, seeded with keratinocytes will be maintained as so-called organotypic cultures for defined periods and cell viability and proliferation will be monitored and optimised.

The project therefore will combine a wide range of cell culture and molecular biological approaches in addition to a sound general training in research.

At present, we would only consider applications from prospective students with:
- a good (bio)medical degree, with interests in any of the areas outlined above,
- good command of the English language as outlined in the postgraduate prospectus,
- a source of funding to cover tuition fees and bench fees.

To be considered for this studentship, please send the following documents to [Email Address Removed]:
• A detailed CV, including your nationality and country of birth;
• Names and addresses of two referees;
• A covering letter highlighting your research experience/capabilities;
• Copies of your degree certificates with transcripts;
• Evidence of your proficiency in the English language, if applicable.