Systematic identification of host virus interactions
Disease outcome from virus infection depends on complex host pathogen interactions. Rational design of therapies, vaccines and disease control depends on understanding these interactions at the molecular level. In the Grey lab we use cutting edge systems biology approaches to identify and characterise host genes involved in virus infection [1-3].
Our work focuses on human cytomegalovirus (HCMV) (although we also work with other viruses). HCMV is a highly prevalent herpesvirus, infecting greater than 30% of the population. Although normally asymptomatic in healthy individuals, HCMV infection is a significant cause of morbidity and mortality in immunocompromised populations, individuals with heart disease and recipients of solid organ and bone marrow transplant. HCMV is also the leading cause of infectious congenital birth defects resulting from spread of the virus to neonates. HCMV also epitomises the complex nature of host pathogen interaction having evolved multiple strategies for subverting host cell functions for the benefit of the virus. The project will combine high throughput screening using lentivirus libraries and siRNA libraries to identify host genes involved in virus infection
The project will provide an opportunity to receive training in basic cell biology and virology techniques as well as more advanced techniques including bioinformatics, deep sequencing, systematic siRNA screening and CRISPR technology.
Applications including a statement of interest and full CV with names and addresses (including email addresses) of two academic referees, should be sent to: Liz Archibald, The Roslin Institute, The University of Edinburgh, Easter Bush, Midlothian, EH25 9RG or emailed to [Email Address Removed].
When applying for the studentship please state clearly the title of the studentship and the supervisor/s in your covering letter.
All applicants should also apply through the University’s on-line application system for September 2016 entry via http://www.ed.ac.uk/studying/postgraduate/degrees/index.php?r=site/view&id=831
International students should also apply for an Edinburgh Global Research Studentship (http://www.ed.ac.uk/schools-departments/student-funding/postgraduate/international/global/research).
ALL APPLICATION PROCEDURES MUST BE COMPLETED BY THE CLOSING DATE 1st FEBRUARY 2016
Grey F, Meyers H, White EA, Spector DH, Nelson J. A Human Cytomegalovirus-Encoded microRNA Regulates Expression of Multiple Viral Genes Involved in Replication. PLoS Pathog. 2007;3(11):e163. PubMed PMID: 17983268.
Grey F, Tirabassi R, Meyers H, Wu G, McWeeney S, Hook L, et al. A viral microRNA down-regulates multiple cell cycle genes through mRNA 5'UTRs. PLoS Pathog. 2010;6(6):e1000967. Epub 2010/06/30. doi: 10.1371/journal.ppat.1000967. PubMed PMID: 20585629; PubMed Central PMCID: PMC2891821.
Pavelin J, Reynolds N, Chiweshe S, Wu G, Tiribassi R, Grey F. Systematic microRNA analysis identifies ATP6V0C as an essential host factor for human cytomegalovirus replication. PLoS Pathog. 2013;9(12):e1003820. Epub 2014/01/05. doi: 10.1371/journal.ppat.1003820. PubMed PMID: 24385903; PubMed Central PMCID: PMC3873435.