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Establishment and analysis of an oral keratinocyte organotypic model


Project Description

Project Aims: To develop an organotypic model of oral mucosa, comparing the suitability of particular donor sites within the oral cavity. To quantitatively evaluate the morphological similarities in vitro and in vivo. To identify the effects of known carcinogens (including tobacco extract and 4NQO) on the morphology and gene expression of oral mucosa in vitro. Initially keratinocytes isolated from different sites of adult and neonate rat oral mucosa will be established in culture grown on a feeder layer of fibroblasts before transferring the keratinocytes to different examples of organotypic cultures and examining the influence of passage number, the site from which samples were isolated and the type of culture on the gene expression and histological behaviour of the keratinocytes. Having established which cultures best represent the in vivo tissues, further cultures will be established and exposed to different conditions to include cigarette smoke extract, a known carcinogen (4NQO), hydrogen peroxide to identify how these conditions influence the behaviour of the keratinocytes using immunohistochemistry, RT-PCR and image analysis of histological sections.

Funding Notes

We have a thriving international Researcher community and encourage applications from students of any nationality able to fund their own studies (i.e. through Government scholarship schemes), or who wish to apply for their own funding (e.g. Commonwealth Scholarships, Islamic Development Bank International PhD Scholarships, China Scholarship Council).

At present, we would only consider applications from prospective students with:
- a 1st. class biomedical degree (or possibly a 2:1)
- a source of funding to cover tuition fees and bench fees.

How good is research at University of Birmingham in Allied Health Professions, Dentistry, Nursing and Pharmacy?

FTE Category A staff submitted: 15.90

Research output data provided by the Research Excellence Framework (REF)

Click here to see the results for all UK universities

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