Gene expression and metal homeostasis in Cyanobacteria (Newcastle)
Cyanobacteria hold a special place in the development of life. They invented oxygenic photosynthesis and now produce 25% of oxygen on the planet. Cyanobacteria recycle substantial proportion of atmospheric carbon dioxide and have a huge potential as producers of biofuels for industry. However, little is known about cyanobacterial gene expression, the most important component for understanding molecular biology of cyanobacteria. This project will investigate the first step of gene expression – transcription – copying of genetic information into RNA. Remarkably, the molecular mechanisms involved in regulation of transcription in cyanobacteria are very different from other bacteria; (i) cyanobacteria lack ubiquitous transcription factors that control fidelity of transcription and continuous movement of RNA polymerase along genomic DNA; (ii) because of ultimate dependency on photosynthesis, they have very different content of metal ions required for accurate transcription; (iii) transcription is controlled by a day-night circadian clock. To investigate these unusual phenomena we will use wide range of genetics, molecular biology, biochemical and biophysical techniques. Global gene expression profiles will be analysed by novel methods based on next generation sequencing with accompanied bioinformatics. The project will be chiefly based in the world-class Centre for Bacterial Cell Biology at Newcastle University. Additionally, the project will benefit greatly from student’s rotation in Durham University in the lab of DTP partner, the leading expert in molecular and cell biology of metals.
For further information see the website: http://www.ncl.ac.uk/camb/
Please submit a full CV and covering letter directly to [email protected]
This is a 4 year BBSRC studentship under the Newcastle-Liverpool-Durham DTP. The successful applicant will receive research costs, tuition fees and stipend (£14,057 for 2015-16). The PhD will start in September 2016. Applicants should have, or be expecting to receive, a 2.1 Hons degree (or equivalent) in a relevant subject. EU candidates must have been resident in the UK for 3 years in order to receive full support. There are 2 stages to the application process.
Nielsen, S.U., Yuzenkova, Y., and Zenkin, N. (2013). Mechanism of RNA polymerase III transcription termination Science, 340: 1577-1580.