• University of Leeds Featured PhD Programmes
  • Carlos III Health Institute Featured PhD Programmes
  • FindA University Ltd Featured PhD Programmes
  • University of Bristol Featured PhD Programmes
  • Medical Research Council, Harwell Featured PhD Programmes
  • University of Leeds Featured PhD Programmes
  • FindA University Ltd Featured PhD Programmes
  • University of Glasgow Featured PhD Programmes
Newcastle University Featured PhD Programmes
Imperial College London Featured PhD Programmes
King’s College London Featured PhD Programmes
King Abdullah University of Science and Technology (KAUST) Featured PhD Programmes
University of Glasgow Featured PhD Programmes

How do airways mucins form a functional protective barrier?

This project is no longer listed in the FindAPhD
database and may not be available.

Click here to search the FindAPhD database
for PhD studentship opportunities
  • Full or part time
    Prof Thornton
  • Application Deadline
    Applications accepted all year round
  • Self-Funded PhD Students Only
    Self-Funded PhD Students Only

Project Description

In normal physiology, the polymeric mucins MUC5AC and MUC5B provide the organizing framework of the airways mucus gel and are major contributors to its properties. In conjunction with the ciliated cells in the airways epithelium, mucus maintains unobstructed lungs that are free from infection.

MUC5AC and MUC5B are stored, pre-formed within secretory granules awaiting a stimulus for secretion. The current thinking is that mucins change their macromolecular form (or mature) from a cross-linked form in the secretory granule to a linear, expanded form upon release into mucus; this transition being essential for the formation of ‘normal’ mucus. However, the mechanism controlling this transition is not known.

This studentship will address the hypothesis that aberrant mucin maturation results in mucus with sub-optimal properties that leads to obstruction of the airways and infection. The studentship will focus on this fundamentally important issue and will provide the student with a training in a range of state-of-the-art methodologies; these include primary epithelial cell culture, advanced polymer imaging using electron microscopy and AFM, mass spectrometry, and glycoprotein purification and characterization.

This work is a vital first step in understanding the packing and unpacking of mucins within secretory granules. It is anticipated that greater understanding of these processes will lead to better understanding of mucus gel formation and may ultimately lead to the development of novel agents to treat obstructive lung disease.

Funding Notes

This project has a Band 2 fee. Details of our different fee bands can be found on our website. For information on how to apply for this project, please visit the Faculty of Biology, Medicine and Health Doctoral Academy website. Informal enquiries may be made directly to the primary supervisor.

References

Thornton, D.J., Rousseau, K. & McGuckin, M. (2008) Structure and function of the polymeric mucins in airways mucus. Annu. Rev. Physiol. 70, 5.1-5.28.


Related Subjects

Share this page:

Cookie Policy    X