Competitive three year full time studentship in the Department of Biological and Medical Sciences
Research Title : Characterisation of golgin function in structural maintenance and biogenesis of the plant Golgi apparatus
Main supervisor Dr Anne Osterrieder
Eligibility:Only open to UK/EU applicants (who must be permanently resident in UK/EU)
Start date: 23rd January 2017
Bursary: £14296 pa (pro rata as January start) for academic year 2016/17 & fees
Closing date: 12th October 2016
Applicants should be of the highest quality and capable of submitting a PhD thesis within 3 years. Requirement a good Honours degree (2.1 or equivalent).
It is preferred that EU applicants have a valid IELTS Academic test certificate minimum score level 6 in each of the four areas of reading, writing, listening and speaking with overall minimum score 7.0 issued since the 23rd April 2015 by an approved test centre please see web site below. Or an undergraduate degree awarded by a recognised UK university within the last two years. https://www.gov.uk/government/publications/guidance-on-applying-for-uk-visa-approved-english-language-tests
Exceptionally we are prepared to consider alternative acceptable written evidence of English language ability.
The Golgi apparatus sits at the heart of the secretory pathway. Secretory cargo enters the Golgi stack for post-translational modification, and is packaged and shipped to its final destination. In plants, the Golgi apparatus also synthesises cell wall material. The plant Golgi apparatus consists of up to hundreds of distinct mobile stacks, meaning that protein trafficking, cell wall material synthesis and Golgi structure need to be maintained during this movement. Golgins are Golgi-located proteins with long coiled-coil domains reaching into the cytoplasm. These domains act as tethers to bind other proteins or membranes. Mammalian golgins have been shown to play a role in the regulation of protein trafficking and Golgi structure, but many of their functions remain unclear in plants.
The aim of this PhD project is to study the role of the golgin AtGolgin-84B in Golgi biogenesis, stack structure maintenance and protein trafficking. The PhD student will receive training in advanced fluorescent live-cell imaging (confocal laser scanning microscopy) and image analysis. Fluorescently tagged Golgin-84B versions will be co-expressed with other Golgi and ER proteins and secretory cargo molecules. The student will also investigate the effects of cytoskeleton- and Golgi-disrupting drugs on fluorescent protein location and dynamics. The second stage of the project aims to identify potential AtGolgin-84B binding partners using the ‘GFP-trap’ method, and to study selected candidate interaction partners using bioinformatics, microscopy and biochemistry. Electron microscopy studies can be carried out in collaboration with Brookes Bioimaging Facility staff.
As part of their Studentship, any successful applicant will be required to undertake up to 6 hours undergraduate teaching/demonstrating a week during semesters on undergraduate practicals without further remuneration. Training will be given.
The appointed individual may have to participate in outreach activities such as Science Festivals and talks in schools.
Any successful applicant may be subject to DBS search once they enrol in view of outreach activities involving schools. In view of checking procedures they will be required to exhibit a current passport - https://www.gov.uk/disclosure-barring-service-check
For further information on the project description only, please contact Dr Anne Osterrieder
e-mail address [email protected]
How to apply
Please complete the Application Form, which you can download from http://www.hls.brookes.ac.uk/images/research/phd-studentship-application-form-jan-14.doc
With your application enclose a CV plus scanned copy of your degree certificates and transcripts and letter from awarding body plus two signed academic references. Additionally if appropriate a valid IELTS Academic test score certificate..
Please carefully note that applications only accepted by e-mail to the following address: [email protected]
YOU MUST NOT SUBMIT ANY QUERIES, APPLICATION, CV OR OTHER DOCUMENTATION VIA FINDAPHD
• Osterrieder A. (2012). Tales of tethers and tentacles: golgins in plants. J. Microsc. 247(1):68-77.
• Schoberer J., Runions J., Steinkellner H., Strasser R., Hawes C., Osterrieder A. (2010). Sequential depletion and acquisition of proteins during Golgi stack disassembly and reformation. Traffic 11(11):1429-44.
• Hawes C., Evans D., Runions J. (2010). A green revolution: getting the measure of a plant cell. Biochemist. 32: 8-11.
• Latijnhouwers, M., Gillespie, T., Boevink, P., Kriechbaumer,V., Hawes, C., Carvalho, C.M. (2007). Localisation and domain characterisation of arabidopsis golgin candidates. J. Exp. Bot. 58:4373-4386.