Unconventional role of a ribosomal subunit in Drosophila embryonic patterning

This project explores the functionality of ribosomes, ancient molecular machines that are responsible for protein production in all living cells. We have recently discovered an interesting Drosophila line with a mutation in a ribosomal subunit that causes defects in the formation of the embryonic gonad. We will explore the function of this ribosomal subunit to solve the apparent paradox between the universal requirement for ribosomes versus the specific defects in this mutant. We will seek to understand whether this subunit is a constitutive component of the ribosome or has extra-ribosomal functions, whether the mutation leads to changes in bulk protein synthesis rates or whether particular proteins are affected and if so, through what mechanism. This work will increase our understanding of the functionalities of the ribosome and may well provide mechanistic insights into ribosomopathies, human disorders of ribosome dysfunction.

Successful candidates will join an active group within the highly rated School of Life Sciences, University of Nottingham. The project will provide excellent training opportunities in recombinant DNA techniques (cloning, PCR), cell culture, Drosophila genetics, microscopy, biochemistry and protein analysis thereby providing comprehensive skills to students embarking on a career in the life sciences. We are based in the Queen’s Medical Centre, Nottingham University, which provides a well-equipped and vibrant scientific community. For more details of our research see germcell.co.uk.