Characterisation and inhibition of novel antimicrobial targets in Pseudomonas aeruginosa

As part of a large collaborative antimicrobial discovery programme we have identified a number of key genes required for full virulence in the bacterial pathogen Pseudomonas aeruginosa (PA). Some of these genes were already known for being central regulators of the quorum sensing regulatory circuits while others still remain to have more precisely their functions and modes of action elucidated. We have seen that the disruption of some of these genes render PA biofilms more susceptible to the action of antibiotics. The aims of this project will be: (i) to identify novel inhibitors of the quorum sensing systems in PA and (ii) to unravel the functions and modes of action of some of the newly identified genes with a view to establish the basis of novel treatments which can eventually be used, in combination with currently available antimicrobials, to treat PA infections. The project will provide extensive training in molecular biology, microscopy and analytical biochemistry techniques.