Dr A Psifidi, Dr R Piercy, Prof D Wells
No more applications being accepted
Competition Funded PhD Project (European/UK Students Only)
About the Project
Department: Clinical Sciences and Services
Duchenne muscular dystrophy (DMD) is a lethal, X-linked, neuromuscular disorder characterised by progressive degeneration of striated muscle, leading to loss of ambulation, respiratory and cardiac failure and death. There are various treatments in use or under development, but there is still no cure. The disease is caused by mutations in the DMD gene that result in a near complete absence of dystrophin (a protein required for normal muscle function) in skeletal and cardiac muscle. Although major progress in defining the pathogenesis has been made, research on understanding the genetic factors contributing to disease severity is at an early stage. In particular, differences in onset and disease progression in different muscles and among individuals suggest that genetic modifiers play a key role.
Taking advantage of the simpler genetic architecture of dogs, and with access to muscle tissues and DNA from affected and control animals, the successful candidate will perform integrated research on DMD, including genome-wide association studies, whole genome sequencing analysis and transcriptomic (RNAseq) profiling in conjunction with pre-existing, extensive and comprehensive longitudinal phenotypic data. The student will learn cutting-edge genetic and bioinformatic technologies and relevant wet lab techniques to identify the underlying genetic and molecular mechanisms that contribute to modifying the DMD phenotype in this novel model, and potentially, their correction via gene therapy or gene editing.
Our findings will facilitate the identification of phenotypic, genomic and molecular biomarkers for DMD providing the means for novel drug discovery.
Interviews will take place in the w/c 2nd or 9th of October
References
Walmsley GL, Arechavala-Gomeza V, Fernandez-Fuente M, Burke MM, Nagel N, Holder A, Stanley R, Chandler K, Marks SL, Muntoni F, Shelton GD, Piercy RJ. A duchenne muscular dystrophy gene hot spot mutation in dystrophin-deficient cavalier king charles spaniels is amenable to exon 51 skipping. PLoS One. 2010 Jan 13;5(1):e8647.
•Vieira NM, Elvers I, Alexander MS.,, Moreira YB., Eran A., Gomes JP., Marshall JL., Karlsson EK., Verjovski-Almeida S, Lindblad-Toh K, Kunkel LM. and Zatz M: Jagged 1 rescues the Duchenne muscular dystrophy phenotype. Cell. 2015 November 19; 163(5): 1204–1213. doi:10.1016/j.cell.2015.10.049
• Vieira NM, Spinazzola JM, Alexander MS, Moreira YB, Kawahara G, Gibbs DE, Mead LC, Verjovski-Almeida S, Zatz M, Kunkel LM. Repression of phosphatidylinositol transfer protein α ameliorates the pathology of Duchenne muscular dystrophy. Proc Natl Acad Sci U S A. 2017 May 22