Is platelet-derived microRNA a modifiable risk factor in patients with acute coronary syndrome?

Micro RNAs (miRNAs) are small non coding RNAs that regulate gene expression post transcription. All blood cells contain significant amounts of miRNA, including human blood platelets where they have been shown to play an important role in platelet production and may be related to platelet reactivity. miRNAs also exist in circulating blood and have major potential as biomarkers in a variety of diseases including coronary artery disease (CAD). This project will explore the relationship between the profiles of miRNAs in platelets and in the plasma of CAD patients to address the following questions:
Do human platelets provide a source of miRNA that is found in the plasma in the form of platelet-derived exosomes?
Do individuals with more active platelets generate higher levels of miRNA contained in exosomes in the circulation?
Is there an increase in platelet-derived exosomes containing miRNA in the circulation of CAD patients and is this affected by antiplatelet medication?
The student will work within a team of other PG students and researchers studying platelet biology and the natural and pathophysiological roles of platelet-derived microvesicles. They would gain experience of a range of state of the art molecular and cell biology techniques including miRNA profiling, RT-PCR, flow cytometry, confocal and electron microscopy, and western blotting, and would become proficient in the study of miRNAs and in the biology of human platelets.


We are an equal opportunities employer and particularly welcome applications for Ph.D. places from women, minority ethnic and other under-represented groups.