Cortical neuronal mechanisms of behavioural and cognitive deficits after sleep deprivation in rodents

A significant portion of our life is spent asleep and we still do not know why. We do know, however, that sleep deprivation leads to behavioural and cognitive impairments, but mechanisms underlying the manifold consequences of sleep loss remain elusive. The overall goal of the project is to elucidate the cortical neuronal mechanisms underlying sensory, behavioural and cognitive deficits that are incurred by sleep deprivation.

The first aim of this project is to investigate wake- and experience-dependent changes in cortical neuronal activity during sleep deprivation at the level of individual neurons, local neuronal assemblies and distributed cortical networks in laboratory rats and mice. The hypothesis will be tested that the changes in the firing patterns of cortical neurons during prolonged waking arise as a result of their preceding activity. The second aim of this project will be to investigate whether and how the changes in cortical neuronal activity during sleep deprivation lead to changes in sensory processing, behaviour and cognition. To this end, visual stimulation, sensory gating and ‘oddball’ paradigms, and spatial navigation and reaction time tasks will be used. These manipulations will be used on one hand to selectively engage and activate specific cortical areas and on the other hand to test the hypothesis that extensive use of specific cortical neurons and/or neuronal assemblies during waking would selectively and progressively depress their function, as would be expected after sleep deprivation.

This project will yield a novel insight into the function of sleep. The student will receive top-class training, and this research will likely lead to publications in high-ranking journals. The student will be working in a cutting-edge laboratory of neurophysiology and will have access to state-of-the art facilities and equipment:

http://www.surrey.ac.uk/biochemistry/People/dr_vladyslav_vyazovskiy/

The successful applicant should have a strong interest in neurosciences, and be highly motivated to perform experiments. Practical experience in working with rodents in vivo (e.g. surgery, behavioural experiments, electrophysiology), good quantitative and programming skills (Matlab) and knowledge in electrical engineering are important. Previous experiences with the visual system, in vitro work, pharmacology and focal viral delivery will be advantageous. Proficiency in the English language, good communication and social skills are important.