Exploring fundamental roles of the cytoskeleton in cellular mechanisms of neurodegeneration

It is well established that cytoskeletal factors such as Tau, Kinesins or Dyneins play crucial roles in neurodegenerative processes. But understanding the role these proteins play in the cellular processes that eventually kill neurons remains a major challenge in the field. An essential road block to advance on this problem is the molecular complexity of cytoskeletal machinery. Overcoming this complexity requires creative new approaches.

We have established Drosophila neurons as a powerful system in which to address the fundamental mechanisms underpinning cytoskeleton functions and regulations. We have clearly demonstrated that knowledge gained in this system translates into mammalian/human biology. The versatile genetic strategies used by us to decipher the actin and microtubule machineries, and the links between them, is unique and unrivalled, providing exciting opportunities to pioneer new avenues of research into this important area of neurobiology.

From the start of your project, you will capitalise on our well established cellular models of neurodegeneration, induced by genetic manipulation of cytoskeletal regulators. These models display relevant features including severe disorganisation of microtubule networks and loss of synapses. During your project you will apply the breadth of molecular tools available in the areas of actin and microtubule regulation or the mechanisms of oxidative stress. We will encourage you to creatively contribute and will support you in developing your individual line of investigation.

You will gain excellent training in modern research techniques, including generation of cell cultures from neural embryonic stem cells or dissociated brains, genetic strategies to dissect molecular mechanisms, state-of-the-art techniques in molecular biology and biochemistry, generation of transgenic animals, drug application, advanced microscopy including live imaging and sophisticated image analysis. Together with transferable skills trained on the postgraduate training programme of Manchester's Faculty of Life Sciences, this project will prepare you for all aspects of your future career.