Proteomics based analysis of RNA virus entry and exit

Paramyxoviruses are a great health and economic burden across the globe resulting in numerous deaths and considerable hardship. Examples from this large family include the well-known human pathogens measles virus (MeV) and respiratory syncytial virus (RSV). These two viruses alone cause a huge number of outbreaks and fatalities each year in the developing world. This family also contains many important viruses of animals as well as dangerous zoonotic pathogens, such as Nipah and Hendra virus.

Paramyxoviruses such as MeV and RSV are enveloped and acquire their membrane from infected cells in a process known as budding. This envelope then fuses with the target cell after attachment to allow the viral genome into the cell. Unfortunately, the interactions involved in both these processes are not fully understood. The aim of this PhD project is therefore to significantly improve our understanding and knowledge of these viral mechanisms. This information can then be used for the rational design of antivirals and vaccines in the future.

This project adopts a multidisciplinary approach to the study of viral entry and exit, leveraging new methods in proteomics, bioinformatics and systems biology to gain a fundamental understanding of the mechanisms involved. The project will focus on applying proteomics technology (such as quantitative proteomics, tandem affinity purification and mass spectrometry) and bioinformatics (mapping and analysing interaction networks) to dissect the mechanisms of virus attachment and release. Small RNA viruses frequently hijack components of the cell in order to drive the production of new virus particles. Isolating, identifying and characterising this hijacked host cell machinery in the context of infected cells will provide the chosen student with the unique opportunity to work with important human pathogens and develop the key virology skills required for a career in research.

In addition to proteomics and bioinformatics, this project will involve the use of confocal and electron microscopy, quantitative PCR, cloning and reverse genetics. The application of these techniques to negative strand RNA viruses represents an exciting and novel area for future research. As such the targets of this project are the publication of peer-reviewed publications in high-impact journals as well as the presentation of data, by the student, at national and international conferences.

Person specification:

Essential: A keen interest in virology and/or systems biology as well as a commitment to research. Applicants should hold, or realistically expect to obtain, at least an Upper Second Class Honours Degree in a relevant subject.

Desirable: Applicants should have a strong background in protein biochemistry/cell/molecular biology. Previous laboratory experience is also desirable, although full training will be provided.

Applications should including a CV, names and addresses of two referees and a covering letter to be sent to Vikki Harrison at [Email Address Removed]
Applicants are also encouraged to make informal enquiries to Dr. Dalan Bailey at [Email Address Removed]

The position will remain open until filled with interviews taking place early July for an October 2013 start.